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Followers
396
Account age
3 yrs
💰 Suggested sponsorship rate
$40 – $65 / sponsored post
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📊 Activity & format
Posting cadence
85.7 / week
A very active account — posts many times a day.
Content mix
Mostly images
Recent: 6 text · 6 image · 0 video.
Follower / following
1.3×
Follows 314 back. A more reciprocal / networked account.
Recent posts
View on Mastodon ↗
Plasmids are already available from the European Plasmid Repository (link) and are on their way to Addgene. Flies will become available from stockcenters and in the meantime directly from us. We are excited to see what you will do with the…
This project has been a real team effort and I would like to thank everyone involved. The majority of authors are actually undergraduates (most doing a gap year between high school and uni in our lab) and the level of skill and enthusiasm …
Also check out the cool recent work by the Hart, Doench (PMID 38678031) and Aguzzi (DOI 10.1101/2022.05.25.493370) labs on multiplex gene targeting in cultured mammalian cells.
Overall, we find that the multiplexed Cas12a system substantially outperforms the systems we have been using so far, enabling us to make new discoveries. We are also very excited about the LOH assay as a new means to screen in an unbiased …
Notably, these phenotypes were not only missed in the Cas9/HD_CFD half of our screen, but also in the literature. There is a nice paper on trem function (PMID* ***21383963**) based on a transposon insertion and does not report wing phenoty…
To confirm that these phenotypes are real we generated additional sgRNA lines. Once you have good lines the phenotype also appears with Cas9-mediated mutagenesis (and even some RNAi lines). We also generated germline mutants in trem, which…
Why does this matter? Because the more efficient system reveals phenotypes previously missed. Example: The gene *trade embargo* (*trem*). Targeting *trem* with Cas9 /HD_CFD does not result in wing phenotypes. But multiplex Cas12a targeting…
We compared the phenotypic effects of targeting 103 genes for which we also have sgRNA lines in the Cas9 HD_CFD library. The multiplexed Cas12a system induced more severe phenotypes for the majority of wing size regulators. 28/n
Gene targeting with Cas12a+ and 4xsgRNA arrays resulted in a clear separation of positive and negative control genes. This is further evidence that this system is specific, well tolerated, and effective. 27/n
For a quantitative comparison of mutagenesis effects across many genes, we measured the organ size of the adult wing as our phenotypic readout. 26/n
In the previous example one can also appreciate another important aspect: Mutagenesis is efficiently confined to the expression domain of the nuclease (dotted line), even with U6 driven sgRNAs. Something we and others have struggled with w…
Another example: Mutagenesis of the Hedgehog signaling component *smoothened*. The multiplexed Cas12a system leads to much more efficient gene disruption. Also note the stronger reduction in size of the mutated area – Smo is important for …
To confirm that these phenotypes are real we generated additional sgRNA lines. Once you have good lines the phenotype also appears with Cas9-mediated mutagenesis (and even some RNAi lines). We also generated germline mutants in trem, which…
Why does this matter? Because the more efficient system reveals phenotypes previously missed. Example: The gene *trade embargo* (*trem*). Targeting *trem* with Cas9 /HD_CFD does not result in wing phenotypes. But multiplex Cas12a targeting…
We compared the phenotypic effects of targeting 103 genes for which we also have sgRNA lines in the Cas9 HD_CFD library. The multiplexed Cas12a system induced more severe phenotypes for the majority of wing size regulators. 28/n
Gene targeting with Cas12a+ and 4xsgRNA arrays resulted in a clear separation of positive and negative control genes. This is further evidence that this system is specific, well tolerated, and effective. 27/n
For a quantitative comparison of mutagenesis effects across many genes, we measured the organ size of the adult wing as our phenotypic readout. 26/n
In the previous example one can also appreciate another important aspect: Mutagenesis is efficiently confined to the expression domain of the nuclease (dotted line), even with U6 driven sgRNAs. Something we and others have struggled with w…
Another example: Mutagenesis of the Hedgehog signaling component *smoothened*. The multiplexed Cas12a system leads to much more efficient gene disruption. Also note the stronger reduction in size of the mutated area – Smo is important for …
🐘 Community & instance
Home server
fediscience.org
Their home server on the fediverse — the instance a creator picks signals the community they belong to.
On Mastodon since
Nov 2022
Joined in the Twitter-exodus wave of late 2022 — part of the migration that made Mastodon a real destination.
💡 Facts
🗓️Joined Mastodon in 2022 — 3 years ago.
📤Posts about 85.7× per week.
🕵️ Fake follower check
Estimated57/100
Fair
Credibility score
89%
Real
Real audience
Low
Fake-follower risk
Medium
Data confidence
- Est. 89% real, active audience · Low fake-follower risk.
- Limited engagement data — score uses the signals available.
- Established account (3+ years old).
Heuristic estimate from engagement, follower ratios, account age & growth — a screening signal, not a guarantee.
About
Scientist at the German Cancer Reseach Center in Heidelberg. We develop genome engineering tools for functional genomics. #CRISPR #Drosophila #openscience
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