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Roby Bhattacharyya

Roby Bhattacharyya

Mastodon @roby@mstdn.science
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🔄 Data last refreshed 13 days ago
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🔒 Paid proposals held safely in escrow — released only when the work's approved.

📣Sponsored postOne disclosed post (toot) with your link and message. $12→ 🧵Sponsored threadA multi-post explainer or story — the fediverse rewards dept… $30→ 📦Product seeding / honest reviewSend me your product — I post a disclosed, honest take. Auth… $16→ 📌Pinned sponsored postA disclosed post pinned to the top of my profile for the mon… $36/mo→ 🔗Profile link (bio field)Your link in one of my profile metadata fields for the month… $18/mo→ 🔁Boost packageI boost (repost) your account's toots through the month — di… $24/mo→ 🏛️Instance / community sponsorshipSponsor the server I run — a sponsor line in the About/rules… $30/mo→ 🤝Mutual boost collabWe boost each other across instances — a value swap between … Swap→ 🎬UGC HireBranded content for your own channels & ads from $50→ 🎙️Podcast guest spotInvite Roby Bhattacharyya onto your podcast (~45 min, remote) $27→ 📞1:1 ConsultPaid video call — advice, coaching or Q&A $75/hr→

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Followers
637
Account age
3 yrs
🎁 Free analysisfor Roby Bhattacharyya
🕵️Fake Followers CheckReal-audience % & bot risk→ 📊Engagement rateBenchmarked vs. peers→ 💰What they chargeFair sponsored-post price→

Known for

8/n …1. Diagnostic implications (cont'd): we realized that ertapenem, which is bulkier, is more affected by porin deficiency than meropenem, whereas both are ~equivalently hydrolyzed by carbapenemases. So we checked, and sure enough, the ratio of ertapenem to meropenem MICs at standard inoculum performs almost as well as the IE at identifying CP-CRE - and since we always measure both erta & mero M3 views 8/n …1. Diagnostic implications (cont'd): we realized that ertapenem, which is bulkier, is more affected by porin deficiency than meropenem, whereas both are ~equivalently hydrolyzed by carbapenemases. So we checked, and sure enough, the ratio of ertapenem to meropenem MICs at standard inoculum performs almost as well as the IE at identifying CP-CRE - and since we always measure both erta & mero M 7/n …1. Diagnostic implications (cont'd): so what can we do to find CP-CRE better? It turns out meropenem MIC is lousy at distinguishing CP-CRE from non-CP-CRE - almost literally a coin flip, since MIC distributions of CP-CRE and non-CP-CRE cross in the CLSI inoculum range. Measuring the IE does an excellent job at finding CP-CRE, but requires an unacceptable amount of extra work for already-too-b2 views 7/n …1. Diagnostic implications (cont'd): so what can we do to find CP-CRE better? It turns out meropenem MIC is lousy at distinguishing CP-CRE from non-CP-CRE - almost literally a coin flip, since MIC distributions of CP-CRE and non-CP-CRE cross in the CLSI inoculum range. Measuring the IE does an excellent job at finding CP-CRE, but requires an unacceptable amount of extra work for already-too-b 4/n …which means these carbapenemases end up shared with neighbors. (In theory they could be constrained to the periplasm, but at least above a certain cell density, this doesn't seem to be the case: all CP-CRE have a strong IE. By contrast, porin deficiency, which prevents carbapenems from getting into the periplasm, is a cell-autonomous resistance mechanism that has no effect on neighbors, hence2 views 4/n …which means these carbapenemases end up shared with neighbors. (In theory they could be constrained to the periplasm, but at least above a certain cell density, this doesn't seem to be the case: all CP-CRE have a strong IE. By contrast, porin deficiency, which prevents carbapenems from getting into the periplasm, is a cell-autonomous resistance mechanism that has no effect on neighbors, hence 5/n So why is this important? We thought of diagnostic, clinical, and mechanistic implications. 1. Clinical diagnostics: we measure resistance at a certain cell density to guide antibiotic use, so having MICs that vary widely is a problem. The IE is known, so guidance is given on acceptable inoculum range ("CLSI range" in figs) - but even in this range, MICs can vary widely. In fact, 1/3 of CP-CRE1 views 5/n So why is this important? We thought of diagnostic, clinical, and mechanistic implications. 1. Clinical diagnostics: we measure resistance at a certain cell density to guide antibiotic use, so having MICs that vary widely is a problem. The IE is known, so guidance is given on acceptable inoculum range ("CLSI range" in figs) - but even in this range, MICs can vary widely. In fact, 1/3 of CP-CRE

📊 Post engagement

3
Avg engagement / post
0.5%
Engagement vs followers
Nov 2022
On Mastodon since

🔥 Top post: Just realized that the https://www.bhattacharyyalab.org/ officia · 7 likes + reposts

📊 Activity & format

Posting cadence
0.85 / week
A lower-frequency account — each post lands with more weight.
Content mix
Mostly text
Recent: 8 text · 4 image · 0 video.
Follower / following
1.9×
Follows 332 back. A more reciprocal / networked account.

Recent posts

View on Mastodon ↗
🔥 Top post Just realized that the https://www.bhattacharyyalab.org/ officially turned 4 on July 1! But I sortof missed the occasion. In my defense, 3 of those years were fairly distracting as an ID doc… ★ 7
3 bhattacharyyalab.org posters today @ #IDWeek2023! #250 PierreAnkomah: kinetics of a novel monocyte subtype in sepsis, implications for immunopathology #623 Eleanor Young: a promising approach to fungal diagnostics #875 David Roach: democ… ★ 5 For those interested, I found the energy to post a thread on this preprint (not sure how this pseudo-quote-toot will work): https://mstdn.science/@roby/110661479775958698 ★ 5 12/12 Great pleasure working with Alex and Kyra on this; they both did an INCREDIBLE amount of hard work to get this story where it was. I think I learned more from the writing & revising process on this project than on any prior paper I'v… ★ 2 11/n Anyway, the clearest way I can summarize what I learned from this project is: the carbapenem inoculum effect depends on the mechanism of resistance of the isolate, not the mechanism of action of the antibiotic. (So we should perhaps c… ★ 1 10/n 3. Mechanistic implications, & musings: thinking this through made me reflect on periplasmic dynamics in beta-lactam resistance. It's all about preventing beta-lactams from reaching their PBP targets, by hydrolysis or low import or bo… ★ 1 9/n That's most of the story. A couple other reflections (see preprint for more): 2. Clinical implications: Porin-deficient strains take up carbapenems less well, but also nutrients, making them less fit. Yet studies haven't shown a consis… ★ 1 8/n …1. Diagnostic implications (cont'd): we realized that ertapenem, which is bulkier, is more affected by porin deficiency than meropenem, whereas both are ~equivalently hydrolyzed by carbapenemases. So we checked, and sure enough, the r… ★ 3 7/n …1. Diagnostic implications (cont'd): so what can we do to find CP-CRE better? It turns out meropenem MIC is lousy at distinguishing CP-CRE from non-CP-CRE - almost literally a coin flip, since MIC distributions of CP-CRE and non-CP-CR… ★ 2 6/n …1. Diagnostic implications (cont'd): These "unstable" MICs in the approved inoculum range mean that we're most likely to misclassify carbapenemase-producing isolates - just the ones we worry about most, bc they can share carbapenemase… ★ 2 5/n So why is this important? We thought of diagnostic, clinical, and mechanistic implications. 1. Clinical diagnostics: we measure resistance at a certain cell density to guide antibiotic use, so having MICs that vary widely is a problem.… ★ 1 4/n …which means these carbapenemases end up shared with neighbors. (In theory they could be constrained to the periplasm, but at least above a certain cell density, this doesn't seem to be the case: all CP-CRE have a strong IE. By contras… ★ 2

🐘 Community & instance

Home server
mstdn.science
Their home server on the fediverse — the instance a creator picks signals the community they belong to.
On Mastodon since
Nov 2022
Joined in the Twitter-exodus wave of late 2022 — part of the migration that made Mastodon a real destination.

💡 Facts

🗓️Joined Mastodon in 2022 — 3 years ago.
👁️Averages 3 views per post.
📤Posts about 0.9× per week.

🕵️ Fake follower check

Estimated
66/100
Good Credibility score
89%
Real Real audience
Low Fake-follower risk
High Data confidence
  • Est. 89% real, active audience · Low fake-follower risk.
  • Engagement (~0.5% of followers engage each post) is around typical for Mastodon.
  • Established account (3+ years old).

Heuristic estimate from engagement, follower ratios, account age & growth — a screening signal, not a guarantee.

About

ID doc (MGH), PI (Broad Institute), antibiotic resistance resister, COVID doc, husband, dad. Coffee, tennis, kindness, science, and sleep enthusiast. Here to learn, as that other place devolves. Views mine. | bhattacharyyalab.org

📸 Gallery

8/n …1. Diagnostic implications (cont'd): we realized that ertapenem, which is bulkier, is more affected by porin deficiency than meropenem, whereas both are ~equivalently hydrolyzed by carbapenemases. So we checked, and sure enough, the ratio of ertapenem to meropenem MICs at standard inoculum performs almost as well as the IE at identifying CP-CRE - and since we always measure both erta & mero M 7/n …1. Diagnostic implications (cont'd): so what can we do to find CP-CRE better? It turns out meropenem MIC is lousy at distinguishing CP-CRE from non-CP-CRE - almost literally a coin flip, since MIC distributions of CP-CRE and non-CP-CRE cross in the CLSI inoculum range. Measuring the IE does an excellent job at finding CP-CRE, but requires an unacceptable amount of extra work for already-too-b 5/n So why is this important? We thought of diagnostic, clinical, and mechanistic implications. 1. Clinical diagnostics: we measure resistance at a certain cell density to guide antibiotic use, so having MICs that vary widely is a problem. The IE is known, so guidance is given on acceptable inoculum range ("CLSI range" in figs) - but even in this range, MICs can vary widely. In fact, 1/3 of CP-CRE 4/n …which means these carbapenemases end up shared with neighbors. (In theory they could be constrained to the periplasm, but at least above a certain cell density, this doesn't seem to be the case: all CP-CRE have a strong IE. By contrast, porin deficiency, which prevents carbapenems from getting into the periplasm, is a cell-autonomous resistance mechanism that has no effect on neighbors, hence

🔀 Audience overlap

Estimated

Estimated shared audience with similar creators — useful for avoiding overlap (or doubling down) when planning a campaign.

konama konama 17% Arleka Arleka 14% Keith Keith 11% Rev.Hans :mstdnca: Rev.Hans :mstdnca: 10% Nathan Olsen Nathan Olsen 10% Eric Engestrom 💙 Eric Engestrom 💙 10%

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Roby Bhattacharyya has 637 followers on Mastodon.

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